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Part:BBa_J42043

Designed by: Steve Selinsky   Group: iGEM06_Michigan   (2006-10-30)


araB complete recomb. plasmid

This plasmid contains the front and rear end of the AraB gene flanking either end of a polylinker. It also contains a kanamycin resistance gene which allows confirmation of homologous recombination onto the chromosome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 1202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1231
    Illegal XhoI site found at 1249
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal AgeI site found at 73
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 148


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